Bioc 471a/571a -applied Molecular Genetics Fall 2001 -dr. Roger Miesfeld Diagnostic Applications of Pcr
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چکیده
Multiplex PCR can be used to simultaneously screen for pathogenic and nonpathogenic strains of E. coli using strain-specific primer pairs. By using at least two strain-specific primer pairs for each organism, and replicate sample reactions, it is possible to reliably detect the presence of >10 genome equivalents. In this example, two food samples were analyzed for the presence of a specific pathogenic strain of E. coli . By using appropriate control samples for the two bacterial strains, it can be seen that food sample 1 is contaminated.
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Bioc 471a/571a -applied Molecular Genetics Fall 2001 -dr. Roger Miesfeld
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